Over last 25 years forensic science has gained tremendous grounds as far as evidential values are concerned due to one particular biological tool that is the analysis of DNA. It has revolutionized forensic investigations. The DNA division of this laboratory is well equipped with modern instruments. DNA analysis has broad application in forensic such as disputed paternity testing, murder, rape, individualization etc. The conventional use of immunological and polymorphic enzyme systems, works best in exclusion cases, but chance of inclusion does not exceed above 99.7%. In DNA profiling the probability of identical pattern between the two randomly selected individuals is in order of 1 in 1014 to 1030, keeping in mind the world’s population which is approximately 5 x 1016. Thus the DNA profile can be considered highly unique (except monozygotic twins).